Methodology for analysis of sugar chains obtained from proteoglycans and glycoproteins

A great deal of efforts has been put into the project to establish highly sensitive, rapid and conventional analytical methods for sugar chains found in proteoglycans and glycoproteins by HPLC, CE, NMR and MS for the last decade. Recently, the utility of ESI- and MALDI-TOF-mass spectrometry for sequential elucidation of sulfated sugar chains has been shown by the unique chemical modification of molecules.

Effects of acidic polysaccharides on immune system

Recently, we established the evaluation system for investigation of activity of polysaccharides on immune system using cell culture system. The system is based on the determination of cytokines productions by dendritic cells and macrophages. We also found that chondroitin sulfates binds to L-selectin. This binding is identical to chondroitin sulfates among glycosaminoglycan family.

Functional study of interacting biomolecules with polyamines

Polyamines (putrescine, spermidine and spermine) play an important role in all organisms. We reported previously polyamines mainly bind to RNA molecules and stimulate several kinds of protein synthesis. Also, spermidine is used for post-translational modification of eukaryotic translation initiation factor 5A (eIF5A) which is an essential protein for cell function. Since there are many interacting biomolecules with polyamines in cells, we focus and study about the function of the ones.

Acrolein and cytotoxic disease

Acrolein which is produced from polyamines is aldehyde of highly toxic compound. Our laboratory showed that stroke patients increased the level of protein-conjugated acrolein (PC-Acro) in plasma. To determine the mechanism of production of acrolein and analyze the metabolite in urine, we study by using photo-induced thrombosis model mice. Also, we focus and study about the correlation with PC-acro levels in the other cytotoxic disease.